Research Associate II, Protein Purification

Cambridge, MA
Full Time
Protein - PE&MS
Entry Level

Repertoire Immune Medicines is a preclinical-stage biotechnology company working to unlock and direct the remarkable power of the human immune system to prevent or treat cancer, as well as autoimmune and infectious disease. The company was founded on the belief that understanding the repertoire of T cell receptor (TCR)-antigen immune synapses that drive health and disease represents one of the greatest opportunities for innovation in medical science. Repertoire scientists created and developed the DECODETM suite of technologies, which allow in-depth characterization of TCR-antigen pairs, and the ability to deploy this information in the form of novel targeted immune medicines to fundamentally reprogram the immune system to kill tumors or induce immune homeostasis.

From its sites in Cambridge, Massachusetts and Zurich, Switzerland, Repertoire’s team of approximately 75 is creating a new pipeline of therapeutic immune medicines enabled from its DECODE discovery platform.  Repertoire is currently using its proprietary platform to discover novel epitopes and TCRs from patients with autoimmune diseases such as type 1 diabetes and multiple sclerosis, creating programmable tolerizing mRNA vaccines to restore immune homeostasis in these indications. In oncology, Repertoire’s decoding of tumor-infiltrating lymphocytes and the antigens they target is enabling development of TCR bispecifics and mRNA cancer vaccines as immune oncology-based therapeutics to treat solid tumors. 

On-site Position in Cambridge, MA

Role Overview
We are seeking a passionate Research Associate II to work in the Protein Engineering and Molecular Sciences team to support production of MHC protein. The ideal candidate will have hands-on experience working with mammalian cell culture or E.coli and protein production. The incumbent will work at the bench to express, purify and QC proteins using biochemical methods such as ELISA, SDS-PAGE, Western, and analytical methods such as FPLC and HPLC. The candidate will be responsible for protein production, data analysis and presenting data to their supervisor and to the team in an effective and timely manner. Interested candidates should have strong organizational, interpersonal, verbal, and written communication skills that will enable them to integrate with our highly cross-functional team.

Key Responsibilities

  • Express recombinant proteins from mammalian and bacterial cultures.
  • Optimize protein refolding conditions from inclusion bodies.
  • Execute large-scale protein purifications using Immobilized metal affinity, ion-exchange, and size exclusion chromatography.
  • Characterize recombinant proteins using SDS-PAGE, WB, protein quantitation, HPLC, ELISA and other biochemical/biophysical assays to determine protein purity and function.
  • Capture primary data and document work as required in Electronic Laboratory Notebook (ELN), maintaining up to date records.
  • Present data routinely to the Protein Sciences group and other multidisciplinary groups.
  • BS/MS in Biochemistry, Biology, Bioengineering, Immunology, or a similar field.
  • 2+ years of academic or biotech/pharma laboratory experience.
  • Experience with recombinant protein expression and purification using mammalian cell lines and/or E. coli.
  • Experience with FPLC systems and with affinity, IEX and SEC protein purification methods is desirable.
  • Experience with general in vitro cell culture/immunology techniques and culture of primary cell lines a plus.
  • Strong organizational and communication skills along with excellent attention to details.
  • Enjoys working in a collaborative, fast-paced and quickly changing environment.
Repertoire is committed towards social responsibility and developing an inclusive culture. Much as the power of the immune system lies in the diversity of T and B cells, our work requires the creativity and ingenuity of a diverse workforce.  We believe in actively pursuing equity in all facets of the work experience at Repertoire.  We will continue to educate ourselves about the inequities and barriers present in our society and take action as a company where we can make a difference.
Repertoire is proud to be an Equal Opportunity Employer.

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